Hotspot

EI144_pgp61

LSC · 6423-7860 · 1438 bp

Back to Lepocinclis

Pi 0.2
MarkerSeek score 82.80
Primer available yes
Label EI144_pgp61

Feature Metadata

label_name
EI144_pgp61
feature_type
gene
region
LSC
length_bp
1438
variable_sites
447
pi
0.2
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI144_pgp61_p1 GTCTTCCGTTGCTCCTAAGG ATCCAGTTAGGCAAGTCCTT 55.0 45.0 58.0 56.1 2346 bp 0.25 34.9
EI144_pgp61_p2 TGCTCCTAAGGGAAGGTCAA ATCCAGTTAGGCAAGTCCTT 50.0 45.0 58.0 56.1 2337 bp 0.25 34.6
EI144_pgp61_p3 TTGCTCCTAAGGGAAGGTCA ATCCAGTTAGGCAAGTCCTT 50.0 45.0 58.0 56.1 2338 bp 0.25 34.6
EI144_pgp61_p4 TCTGTCTTCCGTTGCTCCTA ATCCAGTTAGGCAAGTCCTT 50.0 45.0 58.1 56.1 2349 bp 0.25 34.3
EI144_pgp61_p5 GTCTTCCGTTGCTCCTAAGG AATCCAGTTAGGCAAGTCCTT 55.0 42.9 58.0 56.9 2347 bp 0.25 33.1