Hotspot

EI144_pgp60

LSC · 7808-13500 · 5693 bp

Back to Lepocinclis

Pi 0.326

MarkerSeek score 74.10

Primer available yes

Label EI144_pgp60

Feature Metadata

label_name: EI144_pgp60
feature_type: gene
region: LSC
length_bp: 5693
variable_sites: 2701
pi: 0.326
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp60_p1	GGACTTGCCTAACTGGATTT	TGACGCTGGAAACAAAACTG	45.0	45.0	55.3	57.2	1147 bp	0.25	21.8
EI144_pgp60_p2	GGACTTGCCTAACTGGATTTT	TGACGCTGGAAACAAAACTG	42.9	45.0	56.1	57.2	1147 bp	0.25	20.3
EI144_pgp60_p3	GGACTTGCCTAACTGGATTT	GACGCTGGAAACAAAACTGA	45.0	45.0	55.3	56.9	1146 bp	0.25	19.6
EI144_pgp60_p4	GGACTTGCCTAACTGGATTTTTC	TGACGCTGGAAACAAAACTG	43.5	45.0	57.9	57.2	1147 bp	0.25	18.8
EI144_pgp60_p5	TGTCCCTTATTCTTTTATGCGGA	TGACGCTGGAAACAAAACTG	39.1	45.0	57.9	57.2	725 bp	0.25	18.4