Hotspot

EI144_pgp34

LSC · 38171-39662 · 1492 bp

Back to Lepocinclis

Pi 0.308

MarkerSeek score 83.10

Primer available yes

Label EI144_pgp34

Feature Metadata

label_name: EI144_pgp34
feature_type: gene
region: LSC
length_bp: 1492
variable_sites: 712
pi: 0.308
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp34_p1	CCGGCTAATGATGATTCCGT	GGGCCTATTGCTCCTAAACC	50.0	55.0	57.8	58.0	1944 bp	0.25	48.5
EI144_pgp34_p2	CCGGCTAATGATGATTCCGT	TTGCCTTTCCTGAAGCTGTT	50.0	45.0	57.8	57.9	1979 bp	0.25	48.1
EI144_pgp34_p3	CCGGCTAATGATGATTCCGT	GCTTCTGGTTGTCGAGCTAA	50.0	50.0	57.8	57.9	2007 bp	0.25	48.0
EI144_pgp34_p4	CCGGCTAATGATGATTCCGT	CTTCTGCTTCTGGTTGTCGA	50.0	50.0	57.8	57.8	2012 bp	0.25	47.4
EI144_pgp34_p5	CCGGCTAATGATGATTCCGT	TTCTGCTTCTGGTTGTCGAG	50.0	50.0	57.8	57.8	2011 bp	0.25	47.4