Hotspot

EI144_pgp28

LSC · 43596-44156 · 561 bp

Back to Lepocinclis

Pi 0.435

MarkerSeek score 82.90

Primer available yes

Label EI144_pgp28

Feature Metadata

label_name: EI144_pgp28
feature_type: gene
region: LSC
length_bp: 561
variable_sites: 370
pi: 0.435
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp28_p1	GGATGTAGCGCAGTTTGGTA	CCCGTTGTTTTTGCTTCTCC	50.0	50.0	58.0	57.9	1438-1454 bp	0.5	69.0
EI144_pgp28_p2	CAAGGGTCACAGGTTCGAAT	CCCGTTGTTTTTGCTTCTCC	50.0	50.0	57.8	57.9	1399-1415 bp	0.5	67.8
EI144_pgp28_p3	GGATGTAGCGCAGTTTGGTA	TTGCATCGCCTGTTGATTCT	50.0	45.0	58.0	58.2	1872 bp	0.25	48.5
EI144_pgp28_p4	GGATGTAGCGCAGTTTGGTA	CTTGCATCGCCTGTTGATTC	50.0	50.0	58.0	57.8	1873 bp	0.25	48.4
EI144_pgp28_p5	CCGAAACTTGGAGAGGTGTC	CCCGTTGTTTTTGCTTCTCC	55.0	50.0	58.2	57.9	1370 bp	0.25	47.3