Hotspot

EI144_pgp24

LSC · 45650-46475 · 826 bp

Back to Lepocinclis

Pi 0.287
MarkerSeek score 82.80
Primer available yes
Label EI144_pgp24

Feature Metadata

label_name
EI144_pgp24
feature_type
gene
region
LSC
length_bp
826
variable_sites
367
pi
0.287
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI144_pgp24_p1 TATACCACCTCCACTTGCCT AGCTTGTGAGGTTTGGGAAG 50.0 50.0 57.7 58.0 1473 bp 0.25 47.8
EI144_pgp24_p2 TATACCACCTCCACTTGCCT GAAGCTTGTGAGGTTTGGGA 50.0 50.0 57.7 58.0 1475 bp 0.25 47.7
EI144_pgp24_p3 TATACCACCTCCACTTGCCT GCTTGTGAGGTTTGGGAAGA 50.0 50.0 57.7 58.0 1472 bp 0.25 47.7
EI144_pgp24_p4 TATACCACCTCCACTTGCCT CTGCAGCTTGTGAAGTTTGG 50.0 50.0 57.7 57.9 1810 bp 0.25 46.9
EI144_pgp24_p5 TATACCACCTCCACTTGCCT CGCGAAGGTGGAGATGTTAT 50.0 50.0 57.7 57.8 1862 bp 0.25 46.1