Hotspot

EI144_pgp19

LSC · 50914-52023 · 1110 bp

Back to Lepocinclis

Pi 0.236

MarkerSeek score 83.30

Primer available yes

Label EI144_pgp19

Feature Metadata

label_name: EI144_pgp19
feature_type: gene
region: LSC
length_bp: 1110
variable_sites: 405
pi: 0.236
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp19_p1	ACCACGTTGTAATCCATCGG	TGGTCCGAATCCCGTTAGTA	50.0	50.0	58.0	57.9	1828 bp	0.25	48.7
EI144_pgp19_p2	CCACGTTGTAATCCATCGGT	TGGTCCGAATCCCGTTAGTA	50.0	50.0	58.0	57.9	1827 bp	0.25	48.7
EI144_pgp19_p3	CCAATTTTTCCACCACGACG	TGGTCCGAATCCCGTTAGTA	50.0	50.0	58.0	57.9	2070 bp	0.25	48.5
EI144_pgp19_p4	ACCACGACGATAAGGAGCTA	TGGTCCGAATCCCGTTAGTA	50.0	50.0	58.0	57.9	2059 bp	0.25	48.5
EI144_pgp19_p5	CATTTCCTTCACGTGTTCGC	TGGTCCGAATCCCGTTAGTA	50.0	50.0	58.0	57.9	2195 bp	0.25	48.5