Hotspot

EI144_pgp15

LSC · 53604-55216 · 1613 bp

Back to Lepocinclis

Pi 0.155

MarkerSeek score 84.20

Primer available yes

Label EI144_pgp15

Feature Metadata

label_name: EI144_pgp15
feature_type: gene
region: LSC
length_bp: 1613
variable_sites: 427
pi: 0.155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp15_p1	ACAATACGAGGTGGTTCACG	TGTGACTCCTTTATTCGCGG	50.0	50.0	57.9	58.0	2395-2487 bp	0.75	81.4
EI144_pgp15_p2	ACGAGGTGGTTCACGAAAAA	TGTGACTCCTTTATTCGCGG	45.0	50.0	58.0	58.0	2482 bp	0.25	49.7
EI144_pgp15_p3	ACGAGGTGGTTCACGAAAAA	TCCAAGTCTAATGTCGCGTG	45.0	50.0	58.0	58.0	2142 bp	0.25	49.7
EI144_pgp15_p4	ACGAGGTGGTTCACGAAAAA	ACTCCTTTATTCGCGGGTTC	45.0	50.0	58.0	58.0	2478 bp	0.25	49.6
EI144_pgp15_p5	ACGAGGTGGTTCACGAAAAA	GACTCCTTTATTCGCGGGTT	45.0	50.0	58.0	58.0	2479 bp	0.25	49.6