Hotspot

EI144_pgp08

LSC · 59250-60725 · 1476 bp

Back to Lepocinclis

Pi 0.4

MarkerSeek score 83.60

Primer available yes

Label EI144_pgp08

Feature Metadata

label_name: EI144_pgp08
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 925
pi: 0.4
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp08_p1	CTTCAATCTGGCGTGACGTA	TCTAGCTTTTTGGCGCAAGA	50.0	45.0	58.0	58.1	2682 bp	0.25	49.1
EI144_pgp08_p2	AATCTGGCGTGACGTAATCC	TCTAGCTTTTTGGCGCAAGA	50.0	45.0	58.1	58.1	2678 bp	0.25	48.6
EI144_pgp08_p3	CTTCAATCTGGCGTGACGTA	GGTCGTTTAAATGGGGCAGA	50.0	50.0	58.0	58.2	2298 bp	0.25	48.6
EI144_pgp08_p4	CTTCAATCTGGCGTGACGTA	TCTGGTCGTTTAAATGGGGC	50.0	50.0	58.0	58.2	2301 bp	0.25	48.6
EI144_pgp08_p5	CGCGGCGAAACCAATAAAAT	TCTAGCTTTTTGGCGCAAGA	45.0	45.0	58.1	58.1	2781 bp	0.25	48.3