Hotspot

psbB

LSC · 76518-78044 · 1527 bp

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Pi 0

MarkerSeek score 29.70

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TGCAATAAAGTTACACAGTGTCT	GCGAGAAGCGAAAAATAGCA	34.8	45.0	56.4	57.2	1610 bp	1.0	40.0
psbB_p2	TGCAATAAAGTTACACAGTGTC	GCGAGAAGCGAAAAATAGCA	36.4	45.0	55.0	57.2	1610 bp	1.0	40.0
psbB_p3	TGCAATAAAGTTACACAGTGTCT	GCGAGAAGCGAAAAATAGCAG	34.8	47.6	56.4	58.3	1610 bp	1.0	40.0
psbB_p4	ATGCAATAAAGTTACACAGTGTCT	GCGAGAAGCGAAAAATAGCA	33.3	45.0	56.9	57.2	1611 bp	1.0	40.0
psbB_p5	TCAATGCAATAAAGTTACACAGTGT	GCGAGAAGCGAAAAATAGCA	32.0	45.0	57.7	57.2	1614 bp	1.0	40.0