Hotspot

ndhF

SSC · 114929-117163 · 2235 bp

Back to Leplaea

Pi 0.00418

MarkerSeek score 44.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 14
pi: 0.00418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCTGGCTGGGAATCAAGAAA	AGAACCGGTGAATCAGAAACA	45.0	42.9	56.7	57.5	2318-2326 bp	1.0	58.4
ndhF_p2	ATCTGGCTGGGAATCAAGAA	AGAACCGGTGAATCAGAAACA	45.0	42.9	56.5	57.5	2319-2327 bp	1.0	56.5
ndhF_p3	TCTGGCTGGGAATCAAGAAAA	AGAACCGGTGAATCAGAAACA	42.9	42.9	57.5	57.5	2318-2326 bp	1.0	56.4
ndhF_p4	ATCTGGCTGGGAATCAAGAAA	AGAACCGGTGAATCAGAAACA	42.9	42.9	57.3	57.5	2319-2327 bp	1.0	54.6
ndhF_p5	TCTGGCTGGGAATCAAGAAA	GAACCGGTGAATCAGAAACA	45.0	45.0	56.7	56.0	2317-2325 bp	1.0	54.2