Hotspot

ndhF-rpl32

SSC · 117164-118146 · 983 bp

Back to Leplaea

Pi 0.0089

MarkerSeek score 46.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 983
variable_sites: 13
pi: 0.0089
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.0	58.4	1170-1179 bp	1.0	77.7
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	AAAAAGCTTTCAACGCTGCC	45.0	45.0	57.8	58.4	1172-1181 bp	1.0	76.5
ndhF-rpl32_p3	TGTTGCCGTCGGAAAAAGTA	TTCAACGCTGCCCAATATCC	45.0	50.0	58.0	58.6	1162-1171 bp	1.0	76.2
ndhF-rpl32_p4	CTGGAAGTGGAACGAAAGGT	AAAAAGCTTTCAACGCTGCC	50.0	45.0	57.7	58.4	1123-1132 bp	1.0	76.1
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TTCAACGCTGCCCAATATCC	45.0	50.0	57.8	58.6	1164-1173 bp	1.0	75.0