Hotspot

psbE-petL

LSC · 68443-69881 · 1439 bp

Back to Leplaea

Pi 0.000927

MarkerSeek score 59.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1439
variable_sites: 2
pi: 0.000927
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1586-1590 bp	1.0	70.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1585-1589 bp	1.0	70.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.8	56.9	1509-1513 bp	1.0	69.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1508-1512 bp	1.0	69.7
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.2	56.9	1594-1598 bp	1.0	69.6