Hotspot

petN-psbM

LSC · 30474-31727 · 1254 bp

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Pi 0.00266

MarkerSeek score 59.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 5
pi: 0.00266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTGGGCCGCTTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1404-1405 bp	1.0	67.1
petN-psbM_p2	TTGGGCCGCTTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1403-1404 bp	1.0	67.1
petN-psbM_p3	GGCCGCTTTAATGGTAGTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.7	57.6	1400-1401 bp	1.0	66.4
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1355-1356 bp	1.0	66.2
petN-psbM_p5	GGGCCGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.7	57.6	1401-1402 bp	1.0	63.5