Hotspot

rpoB-trnC

LSC · 28370-29600 · 1231 bp

Back to Leplaea

Pi 0.00164

MarkerSeek score 41.20

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1231
variable_sites: 3
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1301-1311 bp	1.0	78.0
rpoB-trnC_p2	ATTCCCGCATTTCCATCTCC	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1326-1336 bp	1.0	77.0
rpoB-trnC_p3	GCATTTCCATCTCCGAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1295-1305 bp	1.0	74.9
rpoB-trnC_p4	ATTCCCGCATTTCCATCTCC	TTTGTTGATCAGGCGACACC	50.0	50.0	58.0	58.8	1338-1348 bp	1.0	74.7
rpoB-trnC_p5	ACATTCCCGCATTTCCATCT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.6	58.4	1303-1313 bp	1.0	74.5