Hotspot

psbK-psbI

LSC · 8454-8842 · 389 bp

Back to Leplaea

Pi 0.0018

MarkerSeek score 59.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 1
pi: 0.0018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TGGCAAGCTGCTGTAAGTTT	GGGATTACGTCCTGGATCAT	45.0	50.0	58.3	56.5	493-512 bp	1.0	68.5
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTTTTATTCTTCGCGCCCGG	45.0	50.0	58.3	59.6	511-530 bp	1.0	68.2
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GGGATTACGTCCTGGATCATT	45.0	47.6	58.3	57.2	493-512 bp	1.0	66.6
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTTTATTCTTCGCGCCCGG	45.0	52.6	58.3	58.9	510-529 bp	1.0	65.4
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTTTTATTCTTCGCGCCCG	45.0	47.4	58.3	57.0	511-530 bp	1.0	64.8