Hotspot

trnQ-psbK

LSC · 7914-8267 · 354 bp

Back to Leplaea

Pi 0.00753

MarkerSeek score 58.40

Primer available yes

Label trnQ-psbK

Feature Metadata

label_name: trnQ-psbK
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 4
pi: 0.00753
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-psbK_p1	GAATACCGGGACCAAAACCC	CGGGCAATTTGGCGAATAAA	55.0	45.0	58.5	57.7	483 bp	1.0	74.1
trnQ-psbK_p2	GACCAAAACCCGTTGCCTTA	CGGGCAATTTGGCGAATAAA	50.0	45.0	58.7	57.7	474 bp	1.0	73.0
trnQ-psbK_p3	GAATACCGGGACCAAAACCC	TCGGGCAATTTGGCGAATAA	55.0	45.0	58.5	58.5	484 bp	1.0	72.1
trnQ-psbK_p4	ACTCTGGGACGGAAGGATTC	CGGGCAATTTGGCGAATAAA	55.0	45.0	58.8	57.7	511 bp	1.0	71.9
trnQ-psbK_p5	CGAATACCGGGACCAAAACC	CGGGCAATTTGGCGAATAAA	55.0	45.0	58.9	57.7	484 bp	1.0	71.1