Hotspot

trnR-UCG-accD

LSC · 53245-53564 · 320 bp

Back to Lepisorus

Pi 0.0672

MarkerSeek score 82.30

Primer available yes

Label trnR-UCG-accD

Feature Metadata

label_name: trnR-UCG-accD
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 41
pi: 0.0672
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCG-accD_p1	GTTCGAATCCCCACCAACTC	GGTAGCTTCTTCGGGGAAAG	55.0	55.0	58.5	58.0	416-432 bp	1.0	87.1
trnR-UCG-accD_p2	GTTCGAATCCCCACCAACTC	TCAATCCACCAAATTTGCGC	55.0	45.0	58.5	57.9	390-406 bp	1.0	87.0
trnR-UCG-accD_p3	GTTCGAATCCCCACCAACTC	ATCCACCAAATTTGCGCCTA	55.0	45.0	58.5	58.2	387-403 bp	1.0	86.4
trnR-UCG-accD_p4	GTTCGAATCCCCACCAACTC	TCCACCAAATTTGCGCCTAT	55.0	45.0	58.5	58.2	386-402 bp	1.0	86.4
trnR-UCG-accD_p5	GTTCGAATCCCCACCAACTC	TGCGCCTATCTTCAAACCAA	55.0	45.0	58.5	57.8	375-391 bp	1.0	86.1