Hotspot

rps16

LSC · 4953-6070 · 1118 bp

Back to Lepidium

Pi 0.0221

MarkerSeek score 76.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1118
variable_sites: 109
pi: 0.0221
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAGTCTTTCACATCCTCT	TCAATTACACAAACTAGAGGAATGT	45.0	32.0	55.9	56.5	1154-1226 bp	1.0	51.0
rps16_p2	AGAGGAGGAAAGCAAAGAGT	TCAATTACACAAACTAGAGGAATGT	45.0	32.0	56.1	56.5	1200-1264 bp	0.933333	47.0
rps16_p3	AAGAGGAGGAAAGCAAAGAGT	TCAATTACACAAACTAGAGGAATGT	42.9	32.0	56.8	56.5	1201-1265 bp	0.933333	47.0
rps16_p4	AGAGGAGGAAAGCAAAGAGT	TGAATCAATTACACAAACTAGAGGA	45.0	32.0	56.1	56.2	1204-1268 bp	0.933333	47.0
rps16_p5	AGAGGAGGAAAGCAAAGAGT	CAATTACACAAACTAGAGGAATGT	45.0	33.3	56.1	55.1	1199-1263 bp	0.933333	47.0