Hotspot

petA-psbJ

LSC · 61816-62711 · 896 bp

Back to Lepidium

Pi 0.0304
MarkerSeek score 80.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
896
variable_sites
119
pi
0.0304
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAGAAGGTTCA CAGGATTAGGTTCATCCCTGT 36.4 47.6 55.8 56.8 963-1019 bp 0.933333 49.3
petA-psbJ_p2 AGAAACAGTTTGAGAAGGTTCA TCAGGATTAGGTTCATCCCTGT 36.4 45.5 55.8 58.3 964-1020 bp 0.933333 49.3
petA-psbJ_p3 AGAAACAGTTTGAGAAGGTTCA AGCTGGTATTCTTGTGATTGGT 36.4 40.9 55.8 57.7 1016-1072 bp 0.933333 49.3
petA-psbJ_p4 AAGAAACAGTTTGAGAAGGTTCA CAGGATTAGGTTCATCCCTGT 34.8 47.6 56.5 56.8 964-1020 bp 0.933333 49.3
petA-psbJ_p5 AGAAACAGTTTGAGAAGGTTCAA CAGGATTAGGTTCATCCCTGT 34.8 47.6 56.5 56.8 963-1019 bp 0.933333 49.3