Hotspot

ndhC-trnV-GUA

LSC · 49952-50517 · 566 bp

Back to Lepidium

Pi 0.0339

MarkerSeek score 78.20

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 74
pi: 0.0339
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	TGCCAAAACAGGAATAGCACT	GGTTCGAATCCGTATAGCCC	42.9	55.0	58.1	57.9	472-960 bp	1.0	81.8
ndhC-trnV-GUA_p2	TGCCAAAACAGGAATAGCACT	CGGTTCGAATCCGTATAGCC	42.9	55.0	58.1	58.3	473-961 bp	1.0	80.0
ndhC-trnV-GUA_p3	TGCCAAAACAGGAATAGCAC	GGTTCGAATCCGTATAGCCC	45.0	55.0	56.6	57.9	472-960 bp	1.0	79.7
ndhC-trnV-GUA_p4	TGCCAAAACAGGAATAGCACT	CAGGTCTACGGTTCGAATCC	42.9	55.0	58.1	57.8	481-969 bp	0.933333	76.6
ndhC-trnV-GUA_p5	TGCCAAAACAGGAATAGCACT	GCAGGTCTACGGTTCGAATC	42.9	55.0	58.1	58.4	482-970 bp	0.933333	74.7