Hotspot

trnF-UUC-ndhJ

LSC · 47747-48266 · 520 bp

Back to Lepidium

Pi 0.039

MarkerSeek score 81.60

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 71
pi: 0.039
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	619-660 bp	1.0	91.3
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	618-659 bp	1.0	91.3
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	626-667 bp	1.0	90.6
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	627-668 bp	1.0	90.6
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	616-657 bp	1.0	89.7