Hotspot

trnE-GAA-trnT-ACC

LSC · 30227-30970 · 744 bp

Back to Lepidium

Pi 0.0361

MarkerSeek score 81.20

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 97
pi: 0.0361
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	579-967 bp	1.0	90.7
trnE-GAA-trnT-ACC_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	558-946 bp	1.0	90.2
trnE-GAA-trnT-ACC_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	555-943 bp	1.0	90.0
trnE-GAA-trnT-ACC_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	541-929 bp	1.0	89.8
trnE-GAA-trnT-ACC_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	520-908 bp	1.0	89.7