Hotspot

psbM-trnD-GAC

LSC · 28495-29519 · 1025 bp

Back to Lepidium

Pi 0.0306

MarkerSeek score 76.80

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 129
pi: 0.0306
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	GAGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	47.6	66.7	56.4	60.1	1069-1102 bp	1.0	52.0
psbM-trnD-GAC_p2	AGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	45.0	66.7	55.2	60.1	1068-1101 bp	1.0	52.0
psbM-trnD-GAC_p3	GAGAATGAAGAGTGCAGTAGC	GAGCACCGCCCTGTCAAG	47.6	66.7	56.4	60.7	1100-1133 bp	1.0	52.0
psbM-trnD-GAC_p4	GAGAATGAAGAGTGCAGTAGC	GCCCTGTCAAGGCGGAAG	47.6	66.7	56.4	60.7	1093-1126 bp	1.0	52.0
psbM-trnD-GAC_p5	AGAATGAAGAGTGCAGTAGC	GAGCACCGCCCTGTCAAG	45.0	66.7	55.2	60.7	1099-1132 bp	1.0	52.0