Hotspot

trnS-AGC-trnG-GGA

LSC · 7813-8444 · 632 bp

Back to Lepidium

Pi 0.0354

MarkerSeek score 77.90

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 83
pi: 0.0354
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	685-730 bp	1.0	62.4
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	683-728 bp	1.0	62.1
trnS-AGC-trnG-GGA_p3	CCAACGCTTTAGTCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	57.8	57.3	687-732 bp	1.0	61.3
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	685-730 bp	1.0	61.0
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	50.0	36.4	57.8	56.2	686-731 bp	1.0	60.5