Hotspot

trnK-AAA-rps16

LSC · 4302-4952 · 651 bp

Back to Lepidium

Pi 0.0404

MarkerSeek score 78.40

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 103
pi: 0.0404
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	CCTTTCAGGATCAGTCGTGG	ATCTTCGCAAGGTGGGTTTT	55.0	45.0	58.0	58.0	889-1009 bp	1.0	91.8
trnK-AAA-rps16_p2	GCGGATCTGGACGAATTCTT	ATCTTCGCAAGGTGGGTTTT	50.0	45.0	58.1	58.0	853-973 bp	0.866667	86.2
trnK-AAA-rps16_p3	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	949-1069 bp	0.866667	86.1
trnK-AAA-rps16_p4	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	950-1070 bp	0.866667	86.1
trnK-AAA-rps16_p5	CCTTTCAGGATCAGTCGTGG	GGTTTGTTGCTGCCCTTTTT	55.0	45.0	58.0	58.0	1417-1543 bp	0.733333	80.0