Hotspot

ndhD

SSC · 110341-111858 · 1518 bp

Back to Lepechiniella

Pi 0.00198

MarkerSeek score 47.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1518
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGTGGCGTCAATAGGCTTT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	2035-2037 bp	1.0	78.7
ndhD_p2	ATCCGCCGTTTTTCTTCAGT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	2362-2364 bp	1.0	78.5
ndhD_p3	AATTGTGGCGTCAATAGGCT	TGATACCTTGGGACGGATGT	45.0	50.0	57.9	58.1	2037-2039 bp	1.0	78.4
ndhD_p4	ATTGTGGCGTCAATAGGCTT	TGATACCTTGGGACGGATGT	45.0	50.0	57.9	58.1	2036-2038 bp	1.0	78.4
ndhD_p5	CAATTGTGGCGTCAATAGGC	TGATACCTTGGGACGGATGT	50.0	50.0	57.8	58.1	2038-2040 bp	1.0	77.9