Hotspot

ccsA-ndhD

SSC · 110197-110340 · 144 bp

Back to Lepechiniella

Pi 0.0208

MarkerSeek score 62.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 144
variable_sites: 3
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGTGGCGTCAATAGGCTTT	ACCCCGATCTTGTTCTTTCA	45.0	45.0	58.1	56.8	328-330 bp	1.0	70.0
ccsA-ndhD_p2	TTGTGGCGTCAATAGGCTTT	TGGTATGTACCCCGATCTTGT	45.0	47.6	58.1	58.2	336-338 bp	1.0	70.0
ccsA-ndhD_p3	AATTGTGGCGTCAATAGGCT	ACCCCGATCTTGTTCTTTCA	45.0	45.0	57.9	56.8	330-332 bp	1.0	69.7
ccsA-ndhD_p4	ATTGTGGCGTCAATAGGCTT	ACCCCGATCTTGTTCTTTCA	45.0	45.0	57.9	56.8	329-331 bp	1.0	69.7
ccsA-ndhD_p5	ATTGTGGCGTCAATAGGCTT	TGGTATGTACCCCGATCTTGT	45.0	47.6	57.9	58.2	337-339 bp	1.0	69.7