Hotspot

ycf4-cemA

LSC · 57543-58372 · 830 bp

Back to Lepechiniella

Pi 0.0122

MarkerSeek score 67.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 10
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	TGAGAGAGAAACCCACCAGG	55.0	55.0	58.0	58.6	1020-1029 bp	1.0	76.4
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	TGAGAGAGAAACCCACCAGG	50.0	55.0	58.1	58.6	1019-1028 bp	1.0	76.2
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	TGAGAGAGAAACCCACCAGG	50.0	55.0	58.1	58.6	1018-1027 bp	1.0	76.2
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	TGAGAGAGAAACCCACCAGG	45.0	55.0	58.1	58.6	956-965 bp	1.0	76.0
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	TGAGAGAGAAACCCACCAGG	45.0	55.0	57.8	58.6	972-981 bp	1.0	75.5