Hotspot

psbZ-trnG-GCC

LSC · 35023-35305 · 283 bp

Back to Lepechiniella

Pi 0.0111

MarkerSeek score 63.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 3
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	443-451 bp	1.0	80.8
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	444-452 bp	1.0	80.7
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	441-449 bp	1.0	80.4
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	442-450 bp	1.0	80.4
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	ACGGGCAACTATACCAAACC	50.0	50.0	57.8	57.9	500-508 bp	1.0	80.0