Hotspot

trnK-UUU-rps16

LSC · 4144-4906 · 763 bp

Back to Lepechiniella

Pi 0.00268

MarkerSeek score 62.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 2
pi: 0.00268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	57.9	58.2	937-948 bp	1.0	79.4
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	876-887 bp	1.0	78.8
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	875-886 bp	1.0	78.8
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	885-896 bp	1.0	77.8
trnK-UUU-rps16_p5	TGGACGAATCCTTCCCTTCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	928-939 bp	1.0	77.2