Hotspot

trnH-GUG-psbA

LSC · 87-359 · 273 bp

Back to Lepechiniella

Pi 0.0121

MarkerSeek score 63.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 273
variable_sites: 3
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	370-395 bp	1.0	82.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	384-409 bp	1.0	82.2
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	372-397 bp	1.0	82.2
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	383-408 bp	1.0	82.1
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	385-410 bp	1.0	82.1