Hotspot

ycf1

SSC · 125133-130571 · 5439 bp

Back to Lepanthes

Pi 0.012

MarkerSeek score 46.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5439
variable_sites: 65
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAACAAAGAGAAAGATGAAGAA	CTCATCTAAATACATAAGCCTCCCT	26.1	40.0	52.8	57.7	223 bp	1.0	42.2
ycf1_p2	AAAACAAAGAGAAAGATGAAGAA	TCATCTAAATACATAAGCCTCCCT	26.1	37.5	52.8	56.7	222 bp	1.0	42.2
ycf1_p3	AAACAAAGAGAAAGATGAAGAAT	TCTCATCTAAATACATAAGCCTCCC	26.1	40.0	52.5	57.8	223 bp	1.0	42.2
ycf1_p4	AACAAAGAGAAAGATGAAGAATT	TCTCATCTAAATACATAAGCCTCCC	26.1	40.0	52.5	57.8	222 bp	1.0	42.2
ycf1_p5	AAAACAAAGAGAAAGATGAAGAA	TCTCATCTAAATACATAAGCCTCCC	26.1	40.0	52.8	57.8	224 bp	1.0	42.1