Hotspot

atpA

LSC · 9741-11264 · 1524 bp

Back to Lepanthes

Pi 0.00262

MarkerSeek score 46.30

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 4
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	ATTGATATCCTCGGGTCCGT	50.0	50.0	58.0	58.0	2063-2068 bp	1.0	80.8
atpA_p2	CCTAGCCTTCCAAGCTAACG	ATTGATATCCTCGGGTCCGT	55.0	50.0	58.1	58.0	2066-2071 bp	1.0	80.6
atpA_p3	ATTCCCGCTACCCGCTATAT	ATTGATATCCTCGGGTCCGT	50.0	50.0	58.1	58.0	2035-2040 bp	1.0	80.0
atpA_p4	AGCCTTCCAAGCTAACGATG	AATTGAGCGTGAGAGCCAAA	50.0	45.0	58.0	58.1	2531-2536 bp	1.0	79.9
atpA_p5	AGCCTTCCAAGCTAACGATG	AGCCATTGAACAGCTCGAAA	50.0	45.0	58.0	58.1	2361-2366 bp	1.0	79.9