Hotspot

rpl22-rps19

IRb · 84471-84658 · 188 bp

Back to Lepanthes

Pi 0.0167

MarkerSeek score 66.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 188
variable_sites: 3
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CCTCATAAGAACGTCCACGA	CACAGATCGTATGGTCGGTC	50.0	55.0	57.1	57.9	389-397 bp	1.0	73.8
rpl22-rps19_p2	CCTCATAAGAACGTCCACGA	CAGATCGTATGGTCGGTCAC	50.0	55.0	57.1	57.9	387-395 bp	1.0	73.8
rpl22-rps19_p3	TCCTCATAAGAACGTCCACG	CACAGATCGTATGGTCGGTC	50.0	55.0	57.1	57.9	390-398 bp	1.0	73.8
rpl22-rps19_p4	TCCTCATAAGAACGTCCACG	CAGATCGTATGGTCGGTCAC	50.0	55.0	57.1	57.9	388-396 bp	1.0	73.8
rpl22-rps19_p5	CCTCATAAGAACGTCCACGA	GAGACACGCGAGAAACGATA	50.0	50.0	57.1	57.8	332-340 bp	1.0	73.8