Hotspot

psbE-petL

LSC · 63893-65015 · 1123 bp

Back to Lepanthes

Pi 0.0208

MarkerSeek score 66.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 23
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1284-1286 bp	1.0	74.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1280-1282 bp	1.0	74.1
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1282-1284 bp	1.0	74.1
psbE-petL_p4	ACTGACGAATAACCAACCCG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1293-1295 bp	1.0	73.8
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1295-1297 bp	1.0	73.8