Hotspot

petA-psbJ

LSC · 62253-63128 · 876 bp

Back to Lepanthes

Pi 0.0284

MarkerSeek score 73.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 24
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	1013-1157 bp	1.0	76.7
petA-psbJ_p2	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.2	57.3	1014-1158 bp	1.0	76.7
petA-psbJ_p3	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.7	57.3	1011-1155 bp	1.0	76.0
petA-psbJ_p4	GTCCAAGGCCTTCTGTTCTT	CTATTCCGGGTTGGGTTCAT	50.0	50.0	57.7	57.3	1012-1156 bp	1.0	75.9
petA-psbJ_p5	TTACGAGTCCAAGGCCTTCT	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.4	57.3	1017-1161 bp	1.0	75.3