Hotspot

trnF-GAA-ndhJ

LSC · 47364-47508 · 145 bp

Back to Lepanthes

Pi 0.0414

MarkerSeek score 67.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 6
pi: 0.0414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	58.1	57.9	242 bp	1.0	80.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGGCTGGCCTTTACGTAAAG	50.0	50.0	58.1	57.9	243 bp	1.0	80.7
trnF-GAA-ndhJ_p3	CGTTGGGATAGCTCAGTTGG	GGCTGGCCTTTACGTAAAGA	55.0	50.0	58.3	57.9	282 bp	1.0	78.1
trnF-GAA-ndhJ_p4	CGTTGGGATAGCTCAGTTGG	AGGCTGGCCTTTACGTAAAG	55.0	50.0	58.3	57.9	283 bp	1.0	78.1
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	GGCTGGCCTTTACGTAAAGA	50.0	50.0	57.5	57.9	245 bp	1.0	77.5