Hotspot

psaB-psaA

LSC · 38535-38559 · 25 bp

Back to Lepanthes

Pi 0.04

MarkerSeek score 75.50

Primer available yes

Label psaB-psaA

Feature Metadata

label_name: psaB-psaA
feature_type: igs
region: LSC
length_bp: 25
variable_sites: 1
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-psaA_p1	GCCTTGGTTAAACCTTGGGA	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	154 bp	1.0	80.4
psaB-psaA_p2	AGCCTTGGTTAAACCTTGGG	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	155 bp	1.0	80.4
psaB-psaA_p3	TTCGAAGTCATGTGCGGTAG	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	217 bp	1.0	80.2
psaB-psaA_p4	GCCTTGGTTAAACCTTGGGA	TACAAGGACGTGCTGTAGGA	50.0	50.0	58.0	58.1	153 bp	1.0	79.8
psaB-psaA_p5	TTCGAAGTCATGTGCGGTAG	TACAAGGACGTGCTGTAGGA	50.0	50.0	58.0	58.1	216 bp	1.0	79.6