Hotspot

trnT-GGU-psbD

LSC · 31395-32037 · 643 bp

Back to Lepanthes

Pi 0.03

MarkerSeek score 68.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 18
pi: 0.03
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TCTACCAAGGGCTATAGTCA	50.0	45.0	58.3	54.3	664-700 bp	1.0	54.4
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	ATCTACCAAGGGCTATAGTCA	50.0	42.9	58.3	55.0	665-701 bp	1.0	51.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TCTACCAAGGGCTATAGTCAT	50.0	42.9	58.3	55.0	664-700 bp	1.0	51.6
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	AATCTACCAAGGGCTATAGTCA	50.0	40.9	58.3	55.8	666-702 bp	1.0	50.0
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	TCTTTGATAAATCTACCAAGGGCT	50.0	37.5	58.3	57.7	675-711 bp	1.0	49.2