Hotspot

rpoB-trnC-GCA

LSC · 26013-27350 · 1338 bp

Back to Lepanthes

Pi 0.0233

MarkerSeek score 67.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1338
variable_sites: 30
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1437-1486 bp	1.0	78.8
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1461-1510 bp	1.0	77.7
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1474-1523 bp	1.0	76.8
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.4	57.5	1438-1487 bp	1.0	75.5
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	1438-1487 bp	1.0	74.7