Hotspot

atpA-atpF

LSC · 11265-11336 · 72 bp

Back to Lepanthes

Pi 0.125
MarkerSeek score 74.50
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
72
variable_sites
9
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 ACTAATTTCGTCAGCTCGAA GGGTCCGTGGAAGAAATAACT 40.0 47.6 54.9 57.7 129 bp 1.0 49.6
atpA-atpF_p2 ACTAATTTCGTCAGCTCGAA GGGTCCGTGGAAGAAATAAC 40.0 50.0 54.9 56.1 129 bp 1.0 45.6
atpA-atpF_p3 ACTAATTTCGTCAGCTCGAA GGTCCGTGGAAGAAATAACTGA 40.0 45.5 54.9 58.1 128 bp 1.0 45.6
atpA-atpF_p4 TACTAATTTCGTCAGCTCGA GGGTCCGTGGAAGAAATAACT 40.0 47.6 54.0 57.7 130 bp 1.0 45.5
atpA-atpF_p5 TACTAATTTCGTCAGCTCGAA GGGTCCGTGGAAGAAATAACT 38.1 47.6 54.8 57.7 130 bp 1.0 45.5