Hotspot

trnS-GCU-trnG-UCC

LSC · 7665-8585 · 921 bp

Back to Lepanthes

Pi 0.0255

MarkerSeek score 70.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 23
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATGGCAAGACCCCTTAACT	50.0	45.0	58.0	56.7	1028-1038 bp	1.0	72.9
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AATGGCAAGACCCCTTAACT	50.0	45.0	58.0	56.7	1027-1037 bp	1.0	72.9
trnS-GCU-trnG-UCC_p3	AATTGCAAGTGATCAGGGGG	AATGGCAAGACCCCTTAACT	50.0	45.0	58.1	56.7	1102-1112 bp	1.0	71.6
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AAATGGCAAGACCCCTTAACT	50.0	42.9	58.0	57.5	1029-1039 bp	1.0	70.9
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	AAATGGCAAGACCCCTTAACT	50.0	42.9	58.0	57.5	1028-1038 bp	1.0	70.9