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psbK-psbI

LSC · 6968-7327 · 360 bp

Back to Lepanthes

Pi 0.0284

MarkerSeek score 66.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 10
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTGTTTGGCAAGCTGCTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	478-484 bp	1.0	82.0
psbK-psbI_p2	CTTTGTTTGGCAAGCTGCTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	479-485 bp	1.0	82.0
psbK-psbI_p3	AATCGTAGACGTTATGCCGG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	526-532 bp	1.0	81.1
psbK-psbI_p4	AATCGTAGACGTTATGCCGG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	527-533 bp	1.0	81.1
psbK-psbI_p5	CGTTATGCCGGTTATCCCTT	ACGCCCCGGATCATTAGATA	50.0	50.0	57.8	58.1	517-523 bp	1.0	80.2