Hotspot

ndhD

SSC · 112876-114285 · 1410 bp

Back to Leonurus

Pi 0.00331

MarkerSeek score 60.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1410
variable_sites: 7
pi: 0.00331
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACTTTGTGTGAATTGGCGAG	CCACGAACCATTTTCCTTGC	45.0	50.0	56.9	57.9	1619 bp	1.0	71.3
ndhD_p2	CGAGAGCCTGTCGAATCATA	CCACGAACCATTTTCCTTGC	50.0	50.0	56.9	57.9	1603 bp	1.0	71.3
ndhD_p3	CTTTGTGTGAATTGGCGAGAG	CCACGAACCATTTTCCTTGC	47.6	50.0	57.8	57.9	1618 bp	1.0	70.3
ndhD_p4	CTTTGTGTGAATTGGCGAGA	CCACGAACCATTTTCCTTGC	45.0	50.0	56.7	57.9	1618 bp	1.0	69.0
ndhD_p5	TTTGTGTGAATTGGCGAGAG	CCACGAACCATTTTCCTTGC	45.0	50.0	56.7	57.9	1617 bp	1.0	69.0