Hotspot

rpl32-trnL-TAG

SSC · 110826-111552 · 727 bp

Back to Leonurus

Pi 0.0055

MarkerSeek score 62.60

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 727
variable_sites: 6
pi: 0.0055
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	GGGCAGCGTTAAAAGCATTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	855-857 bp	1.0	80.1
rpl32-trnL-TAG_p2	GGGCAGCGTTAAAAGCATTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	856-858 bp	1.0	80.0
rpl32-trnL-TAG_p3	AAAAAGAAGGGGTATCGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	871-873 bp	1.0	79.6
rpl32-trnL-TAG_p4	AAAAAGAAGGGGTATCGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	872-874 bp	1.0	79.5
rpl32-trnL-TAG_p5	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	865-867 bp	1.0	79.1