Hotspot

ycf4-cemA

LSC · 59364-59922 · 559 bp

Back to Leonurus

Pi 0.00119

MarkerSeek score 59.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 1
pi: 0.00119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAAATCCACCAGGGCAGAAA	55.0	50.0	58.1	58.1	723-728 bp	1.0	79.3
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	653-658 bp	1.0	79.2
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.2	58.1	682-687 bp	1.0	78.3
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	656-661 bp	1.0	77.5
ycf4-cemA_p5	CGTTGACCCGTACTGATGAG	TCCACCAGGGCAGAAATACT	55.0	50.0	58.1	58.3	719-724 bp	1.0	77.3