Hotspot

ndhC-trnM-CAT

LSC · 49188-50986 · 1799 bp

Back to Leonurus

Pi 0.00185
MarkerSeek score 60.80
Primer available yes
Label ndhC-trnM-CAT

Feature Metadata

label_name
ndhC-trnM-CAT
feature_type
igs
region
LSC
length_bp
1799
variable_sites
5
pi
0.00185
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAT_p1 GGGGCTAAAATCCCGGAAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 1918-1941 bp 1.0 77.3
ndhC-trnM-CAT_p2 GGGGCTAAAATCCCGGAAAT AGATGCCGGAGTCAATGGTA 50.0 50.0 57.9 58.5 1979-2002 bp 1.0 76.5
ndhC-trnM-CAT_p3 GGGGCTAAAATCCCGGAAAT TAGATGCCGGAGTCAATGGT 50.0 50.0 57.9 58.5 1980-2003 bp 1.0 76.5
ndhC-trnM-CAT_p4 GGGGCTAAAATCCCGGAAAT TGCCGGAGTCAATGGTATCT 50.0 50.0 57.9 58.5 1976-1999 bp 1.0 76.5
ndhC-trnM-CAT_p5 GGGGCTAAAATCCCGGAAAT ATGCCGGAGTCAATGGTATC 50.0 50.0 57.9 57.4 1977-2000 bp 1.0 76.1