Hotspot

psaA-ycf3

LSC · 40817-41537 · 721 bp

Back to Leonurus

Pi 0.000925

MarkerSeek score 60.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 1
pi: 0.000925
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	824-828 bp	1.0	78.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	828-832 bp	1.0	78.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	902-906 bp	1.0	77.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	823-827 bp	1.0	77.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	817-821 bp	1.0	76.8