Hotspot

trnS-TGA-psbZ

LSC · 34715-35040 · 326 bp

Back to Leonurus

Pi 0.00204

MarkerSeek score 41.80

Primer available yes

Label trnS-TGA-psbZ

Feature Metadata

label_name: trnS-TGA-psbZ
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 1
pi: 0.00204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-TGA-psbZ_p1	CAACCACTCAGCCATCTCTC	TACTTGACCAACCGTCTGGA	55.0	50.0	58.0	58.3	453 bp	1.0	77.7
trnS-TGA-psbZ_p2	CAACCACTCAGCCATCTCTC	AGCAAATACGACAGGTACGC	55.0	50.0	58.0	58.4	431 bp	1.0	77.1
trnS-TGA-psbZ_p3	CAACCACTCAGCCATCTCTC	GCAAATACGACAGGTACGCT	55.0	50.0	58.0	58.4	430 bp	1.0	77.1
trnS-TGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	TACTTGACCAACCGTCTGGA	50.0	50.0	57.9	58.3	473 bp	1.0	76.8
trnS-TGA-psbZ_p5	CAACCACTCAGCCATCTCTC	CAACCGTCTGGAGAAGCAAA	55.0	50.0	58.0	58.4	445 bp	1.0	76.8