Hotspot

rps16-trnQ-TTG

LSC · 6028-7043 · 1016 bp

Back to Leonurus

Pi 0.00262

MarkerSeek score 60.40

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 4
pi: 0.00262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1147 bp	1.0	79.1
rps16-trnQ-TTG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1089 bp	1.0	78.6
rps16-trnQ-TTG_p3	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1161 bp	1.0	77.2
rps16-trnQ-TTG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1103 bp	1.0	76.7
rps16-trnQ-TTG_p5	CGTTGCTTTCTACCACATCG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.2	58.0	1139 bp	1.0	73.9